Kolarova H, Nevrelova P, Bajgar R, Jirova D, Kejlova K, Strnad M
Department of Medical Biophysics, Palacky University, Olomouc, Czech Republic.
Toxicol In Vitro. 2007 Mar;21(2):249-53. doi: 10.1016/j.tiv.2006.09.020. Epub 2006 Oct 1.
Photodynamic therapy (PDT) is a new treatment modality of tumours. The photochemical interactions of sensitizer, light, and molecular oxygen produce singlet oxygen and other forms of active oxygen, such as peroxide, hydroxyl radical and superoxid anion. Phthalocyanine ClAlPcS(2), belonging among the promising second generation of sensitizers, was tested as an inducer of photodamage. We report the production of reactive oxygen species (ROS) and the phototoxicity of ClAlPcS(2) assessed using G361 melanoma cells. A semiconductor laser (lambda=675nm, output power 21mW) was used as a source for evocation of the photodynamic effect. ROS generation and H(2)O(2) release after PDT on G361 cells were detected using probe CM-H(2)DCFDA and recorded by luminescence spectrometer. Viability studies show, that the optimum phototoxic effect tested on G361 melanoma cells was determined in the combination of laser dose of 25Jcm(-2) and phthalocyanine ClAlPcS(2) concentration of 5microg/ml. This combination of phthalocyanine concentration and corresponding radiation dose was lethal for melanoma cells.
光动力疗法（PDT）是一种新型肿瘤治疗方式。敏化剂、光和分子氧之间的光化学相互作用会产生单线态氧以及其他形式的活性氧，如过氧化物、羟基自由基和超氧阴离子。作为第二代有前景的敏化剂之一的氯铝酞菁（ClAlPcS(2)），被作为光损伤诱导剂进行了测试。我们报告了使用G361黑色素瘤细胞评估的活性氧（ROS）的产生以及ClAlPcS(2)的光毒性。使用半导体激光器（波长λ = 675nm，输出功率21mW）作为激发光动力效应的光源。使用探针CM-H(2)DCFDA检测PDT作用于G361细胞后ROS的产生和H(2)O(2)的释放，并通过发光光谱仪进行记录。活力研究表明，在激光剂量为25J/cm(-2)和氯铝酞菁ClAlPcS(2)浓度为5μg/ml的组合下，对G361黑色素瘤细胞测试的最佳光毒性效应得以确定。这种酞菁浓度与相应辐射剂量的组合对黑色素瘤细胞具有致死性。
