Masuko S, Kawasoe M, Chiba T, Shin T
Department of Anatomy, Saga Medical School, Japan.
Neurosci Res. 1991 Jan;9(4):270-8. doi: 10.1016/0168-0102(91)90028-w.
The distribution and origin of peptide-containing intrinsic nerve fibers within the larynx were examined by immunohistochemistry and denervation experiments in the dog. In the normal larynx, a dense network of vasoactive intestinal polypeptide (VIP)-immunoreactive (IR) fibers was seen around the acini of submucosal glands. VIP-, substance P (SP)-, or calcitonin gene-related peptide (CGRP)-IR fibers were seen in the walls of submucosal arteries, and VIP-, neuropeptide Y (NPY)-, or enkephalin (ENK)-IR fibers were seen around the arteries in the muscle tissue. Most of these peptide-IR fibers remained after bilateral denervation of the superior and inferior laryngeal nerves. Several small intrinsic ganglia were found along the peripheral branches of the laryngeal nerves. About 97% of the ganglionic neurons were VIP-IR; of these, 44% were immunoreactive to VIP alone, 22% to VIP and NPY, 13% to VIP and SP, 7% to VIP and ENK, and 14% to VIP, NPY and SP. These results reveal that the exocrine glands and blood vessels are innervated by the intrinsic ganglionic neurons and that subpopulations of ganglionic neurons with different chemical codes innervate specific target organs in the canine larynx.
通过免疫组织化学和去神经实验，对犬喉内含有肽的固有神经纤维的分布和起源进行了研究。在正常喉中，在粘膜下腺泡周围可见密集的血管活性肠多肽（VIP）免疫反应性（IR）纤维网络。在粘膜下动脉壁可见VIP、P物质（SP）或降钙素基因相关肽（CGRP）免疫反应性纤维，在肌肉组织的动脉周围可见VIP、神经肽Y（NPY）或脑啡肽（ENK）免疫反应性纤维。双侧喉上神经和喉下神经去神经后，这些肽免疫反应性纤维大多保留。沿喉神经外周分支发现了几个小的固有神经节。约97%的神经节神经元为VIP免疫反应性；其中，44%仅对VIP有免疫反应，22%对VIP和NPY有免疫反应，13%对VIP和SP有免疫反应，7%对VIP和ENK有免疫反应，14%对VIP、NPY和SP有免疫反应。这些结果表明，外分泌腺和血管由固有神经节神经元支配，并且具有不同化学编码的神经节神经元亚群支配犬喉中的特定靶器官。
