从拟南芥中分离叶绿体蛋白用于蛋白质组分析。
Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis.
作者信息
van Wijk Klaas J, Peltier Jean-Benoit, Giacomelli Lisa
机构信息
Department of Plant Biology, Cornell University, Ithaca, New York, USA.
出版信息
Methods Mol Biol. 2007;355:43-8. doi: 10.1385/1-59745-227-0:43.
PMID:17093301
Abstract
This chapter describes a simple protocol for large-scale chloroplast purification for proteome analysis. The protocol has not been tested for protein import activity but is optimized for chloroplast proteome yield and purity and minimal protein degradation.
摘要
本章介绍了一种用于蛋白质组分析的大规模叶绿体纯化的简单方案。该方案尚未针对蛋白质导入活性进行测试，但已针对叶绿体蛋白质组产量、纯度及最小化蛋白质降解进行了优化。
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