Miernyk Ján A, Preťová Anna, Olmedilla Adela, Klubicová Katarína, Obert Bohuš, Hajduch Martin
Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic.
Sex Plant Reprod. 2011 Mar;24(1):9-22. doi: 10.1007/s00497-010-0149-5. Epub 2010 Sep 10.
While a relative latecomer to the postgenomics era of functional biology, the application of mass spectrometry-based proteomic analysis has increased exponentially over the past 10 years. Some of this increase is the result of transition of chemists, physicists, and mathematicians to the study of biology, and some is due to improved methods, increased instrument sensitivity, and better techniques of bioinformatics-based data analysis. Proteomic Biological processes are typically studied in isolation, and seldom are efforts made to coordinate results obtained using structural, biochemical, and molecular-genetic strategies. Mass spectrometry-based proteomic analysis can serve as a platform to bridge these disparate results and to additionally incorporate both temporal and anatomical considerations. Recently, proteomic analyses have transcended their initial purely descriptive applications and are being employed extensively in studies of posttranslational protein modifications, protein interactions, and control of metabolic networks. Herein, we provide a brief introduction to sample preparation, comparison of gel-based versus gel-free methods, and explanation of data analysis emphasizing plant reproductive applications. We critically review the results from the relatively small number of extant proteomics-based analyses of angiosperm reproduction, from flowers to seedlings, and speculate on the utility of this strategy for future developments and directions.
虽然在功能生物学的后基因组学时代起步相对较晚，但基于质谱的蛋白质组学分析在过去10年中呈指数级增长。这种增长部分是由于化学、物理和数学领域的研究人员转向生物学研究，部分是由于方法改进、仪器灵敏度提高以及基于生物信息学的数据分析技术更好。蛋白质组学的生物学过程通常是孤立研究的，很少有人努力协调使用结构、生化和分子遗传学策略获得的结果。基于质谱的蛋白质组学分析可以作为一个平台，弥合这些不同的结果，并额外纳入时间和解剖学方面的考虑因素。最近，蛋白质组学分析已经超越了最初纯粹的描述性应用，广泛应用于翻译后蛋白质修饰、蛋白质相互作用和代谢网络控制的研究。在此，我们简要介绍样品制备、基于凝胶和非凝胶方法的比较，并重点解释强调植物生殖应用的数据分析。我们批判性地回顾了从花到幼苗的被子植物生殖相对较少的现有蛋白质组学分析结果，并推测这种策略对未来发展和方向的实用性。
