Rabilloud Thierry, Luche Sylvie, Santoni Véronique, Chevallet Mireille
Laboratoire d'Immunologie, CEA, Grenoble, France.
Methods Mol Biol. 2007;355:111-9. doi: 10.1385/1-59745-227-0:111.
Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins. Unfortunately, severe solubility problems hamper the analysis of many classes of proteins, but especially membrane proteins. These problems arise mainly in the extraction and isoelectric focusing steps, and solutions are sought to improve protein solubility under the conditions prevailing during isoelectric focusing. These solutions deal mainly with chaotropes and new detergents, which are both able to enhance protein solubility. The input of these compounds in proteomics analysis of membrane proteins is discussed, as well as future directions.
由于二维电泳对完整蛋白质具有出色的分离能力，若能将其应用于所有类型的蛋白质将是十分有利的。不幸的是，严重的溶解性问题阻碍了许多类蛋白质的分析，尤其是膜蛋白。这些问题主要出现在提取和等电聚焦步骤中，人们正在寻找解决方案以改善等电聚焦过程中蛋白质的溶解性。这些解决方案主要涉及离液剂和新型去污剂，它们都能够提高蛋白质的溶解性。本文讨论了这些化合物在膜蛋白蛋白质组学分析中的应用以及未来的发展方向。
