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阴道微生物组技术洞察

An Insight into Vaginal Microbiome Techniques.

作者信息

Sharma Mahima, Chopra Chitrakshi, Mehta Malvika, Sharma Varun, Mallubhotla Sharada, Sistla Srinivas, Sistla Jyothi C, Bhushan Indu

机构信息

School of Biotechnology, Shri Mata Vaishno Devi University, Katra 182320, India.

Ancient DNA Laboratory, Birbal Sahni Institute of Palaeosciences, Lucknow 226007, India.

出版信息

Life (Basel). 2021 Nov 13;11(11):1229. doi: 10.3390/life11111229.

Abstract

There is a unique microbial community in the female lower genital tract known as the vaginal microbiota, which varies in composition and density and provides significant benefits during pregnancy, reproductive cyclicity, healthy newborn delivery, protection from preterm birth, infections such as UTIs, bacterial vaginosis, and so on, and improves the efficacy of treatments for vaginal cancers. It is necessary to know how the vaginal microbiome is composed in order to make an accurate diagnosis of the diseases listed above. A microbiome's members are difficult to classify, and the way microbial communities function and influence host-pathogen interactions are difficult to understand. More and more metagenomic studies are able to unravel such complexities due to advances in high-throughput sequencing and bioinformatics. When it comes to vaginal microbiota research, we'll be looking at the use of modern techniques and strategies that can be used to investigate variations in vaginal microbiota in order to detect diseases earlier, better treat vaginal disorders, and boost women's health. The discussed techniques and strategies may improve the treatment of vaginal disorders and may be beneficial for women's overall health.

摘要

女性下生殖道存在一种独特的微生物群落,称为阴道微生物群,其组成和密度各不相同,在孕期、生殖周期、健康新生儿分娩、预防早产、预防诸如尿路感染、细菌性阴道病等感染方面具有显著益处,还能提高阴道癌的治疗效果。为了准确诊断上述疾病,有必要了解阴道微生物群的组成。微生物群的成员难以分类,微生物群落的功能方式以及对宿主与病原体相互作用的影响也难以理解。由于高通量测序和生物信息学的进展,越来越多的宏基因组学研究能够揭示此类复杂性。在阴道微生物群研究方面,我们将着眼于运用现代技术和策略来调查阴道微生物群的变化,以便更早地发现疾病、更好地治疗阴道疾病并促进女性健康。所讨论的技术和策略可能会改善阴道疾病的治疗,对女性的整体健康可能有益。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0927/8623751/ac3034875356/life-11-01229-g001.jpg

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