Turrigiano G G, Selverston A I
Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
J Comp Neurol. 1991 Mar 1;305(1):164-76. doi: 10.1002/cne.903050115.
The distribution of cholecystokinin-like immunoreactivity was studied in the stomatogastric nervous systems, pericardial organs, and haemolymph of four species of decapod crustacea, by using immunocytochemical and radioimmunoassay techniques. Whereas cholecystokinin-like immunoreactivity was found within the stomatogastric nervous systems of all four species, its distribution in each is unique. Two species (Panulirus interruptus and Homarus americanus) have cholecystokinin-like immunoreactivity within fibers and neuropil of the stomatogastric ganglion (STG); two other species (Cancer antenarius and Procambarus clarkii) do not. Further, the cholecystokinin-like immunoreactivity within the STGs of Panulirus and Homarus arise from distinct structures; from a projection of anterior ganglia in Panulirus, and from somata within the posterior motor nerves in Homarus. The staining in the other ganglia of the stomatogastric nervous system also shows some interspecies variability, although it appears to be more highly conserved than staining within the STG. These differences in staining were confirmed by measuring the amount of CCK-like peptide present in tissue extracts of ganglia by radioimmunoassay. In contrast to the variable staining within the STG, all four species have cholecystokinin-like immunoreactivity within the neurosecretory pericardial organs and thoracic segmental nerves. This cholecystokinin-like immunoreactivity is contained within fibers and within varicosities that coat the surface of these structures. The location of this staining and the presence of detectible levels of CCK-like peptide in the haemolymph suggests that CCK-like peptides in decapod crustacea may be utilized as neurohormones.
运用免疫细胞化学和放射免疫分析技术，研究了四种十足目甲壳动物的口胃神经系统、围心器官和血淋巴中胆囊收缩素样免疫活性物质的分布情况。尽管在所有四种动物的口胃神经系统中都发现了胆囊收缩素样免疫活性物质，但其在每种动物中的分布都是独特的。两种动物（中断沟龙虾和美洲螯龙虾）在口胃神经节（STG）的纤维和神经毡内有胆囊收缩素样免疫活性物质；另外两种动物（触角黄道蟹和克氏原螯虾）则没有。此外，沟龙虾和螯龙虾的口胃神经节内的胆囊收缩素样免疫活性物质来源于不同的结构；沟龙虾的来源于前神经节的投射，而螯龙虾的来源于后运动神经内的胞体。口胃神经系统其他神经节中的染色也显示出一些种间差异，尽管它似乎比口胃神经节内的染色更具保守性。通过放射免疫分析测量神经节组织提取物中CCK样肽的含量，证实了这些染色差异。与口胃神经节内变化的染色情况不同，所有四种动物在神经分泌性围心器官和胸节段神经内都有胆囊收缩素样免疫活性物质。这种胆囊收缩素样免疫活性物质存在于纤维和覆盖这些结构表面的膨体内。这种染色的位置以及血淋巴中可检测到的CCK样肽的存在表明，十足目甲壳动物中的CCK样肽可能被用作神经激素。
