Matsumura Takayoshi, Suzuki Toru, Kada Nanae, Aizawa Kenichi, Munemasa Yoshiko, Nagai Ryozo
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Biochem Biophys Res Commun. 2006 Dec 29;351(4):965-71. doi: 10.1016/j.bbrc.2006.10.137. Epub 2006 Nov 3.
Protein profiling would aid in better understanding the pathophysiology of metabolic disease. Here, we report on differential proteomic analysis using an animal model of diabetes mellitus and associated metabolic disorders (Otsuka Long-Evans Tokushima Fatty rat). Serum was analyzed by a new two-dimensional liquid chromatography system which separated proteins by chromatofocusing and subsequent reversed-phase chromatography. This is the first application of this approach to differential serum proteomics. Differentially expressed proteins, identified with MALDI-TOF mass spectrometry, included apolipoproteins and alpha2-HS-glycoprotein. These findings add to our understanding of the underlying pathophysiology. This new proteomic analysis is a promising tool to elucidate disease mechanisms.
蛋白质谱分析将有助于更好地理解代谢性疾病的病理生理学。在此，我们报告了使用糖尿病及相关代谢紊乱动物模型（大冢长- Evans 德岛肥胖大鼠）进行的差异蛋白质组学分析。血清通过一种新的二维液相色谱系统进行分析，该系统通过色谱聚焦和随后的反相色谱对蛋白质进行分离。这是该方法首次应用于差异血清蛋白质组学。通过基质辅助激光解吸电离飞行时间质谱鉴定出的差异表达蛋白质包括载脂蛋白和α2 - HS - 糖蛋白。这些发现增进了我们对潜在病理生理学的理解。这种新的蛋白质组学分析是阐明疾病机制的一种有前景的工具。
