Trinchella Francesca, Riggio Marilisa, Filosa Silvana, Volpe Maria Grazia, Parisi Elio, Scudiero Rosaria
Dipartimento delle Scienze Biologiche-Sezione di Biologia Evolutiva e Comparata, Università Federico II, via Mezzocannone 8, 80134 Napoli, Italy.
Comp Biochem Physiol C Toxicol Pharmacol. 2006 Nov;144(3):272-8. doi: 10.1016/j.cbpc.2006.09.004. Epub 2006 Oct 3.
The present report is an attempt to investigate the influence of intraperitoneal and dietary cadmium exposure on the distribution of cadmium accumulation and induction of metallothionein gene expression in different tissues of the lizard Podarcis sicula. Cadmium accumulation in liver, kidney, ovary, brain and intestine was measured by atomic absorption spectrometry. Metallothionein gene induction was determined by dot blot analyses on the total RNA extracted from the same organs. Our data indicate that cadmium exposure results in significant cadmium uptake, but the patterns of this uptake varies with organ and exposure route. After a single intraperitoneal treatment, concentrations of cadmium and metallothionein transcript are positively correlated in kidney, liver and ovary. Following a dietary cadmium treatment, a positive correlation between the increase of metallothionein mRNA and cadmium accumulation is found in intestine, ovary and kidney, while no correlation is present in liver and brain.
本报告旨在研究腹腔注射和饮食中镉暴露对意大利壁蜥（Podarcis sicula）不同组织中镉积累分布以及金属硫蛋白基因表达诱导的影响。通过原子吸收光谱法测定肝脏、肾脏、卵巢、大脑和肠道中的镉积累量。通过对从相同器官提取的总RNA进行斑点印迹分析来确定金属硫蛋白基因的诱导情况。我们的数据表明，镉暴露会导致显著的镉摄取，但这种摄取模式因器官和暴露途径而异。单次腹腔注射处理后，肾脏、肝脏和卵巢中镉浓度与金属硫蛋白转录本呈正相关。饮食镉处理后，肠道、卵巢和肾脏中金属硫蛋白mRNA增加与镉积累之间呈正相关，而肝脏和大脑中则无相关性。
