Williams-Smith D L, Patel K
Biochim Biophys Acta. 1975 Oct 20;405(2):243-52. doi: 10.1016/0005-2795(75)90091-4.
The EPR spectra of bovine liver catalase, rat liver catalase and human erythrocyte catalase have been measured at 9.0 degrees K. In N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES) and Tris buffers at pH 7.0, the liver catalases show EPR spectra typical of rhombically distorted high spin ferric heme with major lines at g = 6.50, 5.35, 1.98. A number of extra lines are also seen; these are weak or absent in human erythrocyte catalase. The effect of the addition of formate, nitrite, acetate, fluoride, azide, hypophosphite and of inactivation with 3-amino-1,2,4-triazole on the degree of rhombic distortion has been studied. There is a good correlation between the low temperature EPR and room temperature optical changes for the binding of formic acid in HEPES and Tris. There is no evidence from EPR spectra for the presence of heme-heme interactions in the binding of formic acid to human erythrocyte catalase. The properties of catalase are altered in phosphate and in distilled water. This is a consequence of the low temperature of measurement.
已在9.0开尔文温度下测量了牛肝过氧化氢酶、大鼠肝过氧化氢酶和人红细胞过氧化氢酶的电子顺磁共振（EPR）光谱。在pH 7.0的N-2-羟乙基哌嗪-N'-2-乙磺酸（HEPES）和Tris缓冲液中，肝脏过氧化氢酶显示出典型的菱形畸变高自旋铁血红素的EPR光谱，主要谱线位于g = 6.50、5.35、1.98处。还观察到许多额外的谱线；这些在人红细胞过氧化氢酶中较弱或不存在。研究了添加甲酸盐、亚硝酸盐、乙酸盐、氟化物、叠氮化物、次磷酸盐以及用3-氨基-1,2,4-三唑灭活对菱形畸变程度的影响。对于HEPES和Tris中甲酸的结合，低温EPR与室温光学变化之间存在良好的相关性。从EPR光谱中没有证据表明在甲酸与人红细胞过氧化氢酶结合时存在血红素-血红素相互作用。过氧化氢酶的性质在磷酸盐和蒸馏水中会发生改变。这是测量温度较低的结果。
