Blum H, Chance B, Litchfield W J
Biochim Biophys Acta. 1978 Jun 21;534(2):317-21. doi: 10.1016/0005-2795(78)90014-4.
Two major rhombic high-spin ferric heme signals are observed during the pH titration of bovine liver catalase. The less rhombic signal if dominant above pH 6.0 and the more rhombic signal below pH 6.0. Ethanol in high concentration enhances the relative intensity of the less rhombic signal. These data demonstrate the sensitivitiy of the ligand field to changes in catalase solvent and, furthermore, suggest that both rhombic configuration posses identical spectral and catalytic properties.
在牛肝过氧化氢酶的pH滴定过程中观察到两个主要的菱形高自旋铁血红素信号。菱形程度较小的信号在pH 6.0以上占主导，而菱形程度较大的信号在pH 6.0以下占主导。高浓度乙醇会增强菱形程度较小信号的相对强度。这些数据证明了配体场对过氧化氢酶溶剂变化的敏感性，此外，还表明两种菱形构型具有相同的光谱和催化特性。
