Ching Paula Carmela O, Chen Fang-Hsu, Lin I-Hsuan, Tran Duong-Thuy, Tayo Lemmuel L, Yeh Ming-Long
Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
School of Chemical, Biological, and Materials Engineering and Sciences, Mapua University, Manila 1002, Philippines.
ACS Omega. 2024 Jul 27;9(31):33629-33642. doi: 10.1021/acsomega.4c01927. eCollection 2024 Aug 6.
The articular cartilage has poor intrinsic healing potential, hence, imposing a great challenge for articular cartilage regeneration in osteoarthritis. Tissue regeneration by scaffolds and bioactive materials has provided a healing potential for degenerated cartilage. In this study, decellularized cartilage powder (DCP) and hyaluronic acid hydrogel modified by aldehyde groups and methacrylate (AHAMA) were fabricated and evaluated in vitro for efficacy in articular cartilage regeneration. In vitro tests such as cell proliferation, cell viability, and cell migration showed that DCP/AHAMA has negligible cytotoxic effects. Furthermore, it could provide an enhanced microenvironment for infrapatellar fat pad stem cells (IFPSCs). Mechanical property tests of DCP/AHAMA showed suitable adhesive and compressive strength. IFPSCs under three-dimensional (3D) culture in DCP/AMAHA were used to assess their ability to proliferate and differentiate into chondrocytes using normal and chondroinductive media. Results exhibited increased gene expression of COL2 and ACN and decreased COL1 expression. DCP/AHAMA provides a microenvironment that recapitulates the biomechanical properties of the native cartilage, promotes chondrogenic differentiation, blocks hypertrophy, and demonstrates applicability for cartilage tissue engineering and the potential for clinical biomedical applications.
关节软骨的自身愈合潜力较差，因此，这对骨关节炎中的关节软骨再生构成了巨大挑战。利用支架和生物活性材料进行组织再生为退化的软骨提供了愈合潜力。在本研究中，制备了脱细胞软骨粉（DCP）和经醛基和甲基丙烯酸酯修饰的透明质酸水凝胶（AHAMA），并在体外评估了它们在关节软骨再生中的功效。细胞增殖、细胞活力和细胞迁移等体外试验表明，DCP/AHAMA具有可忽略不计的细胞毒性作用。此外，它可以为髌下脂肪垫干细胞（IFPSC）提供一个增强的微环境。DCP/AHAMA的力学性能测试显示出合适的粘附力和抗压强度。使用正常培养基和软骨诱导培养基，对在DCP/AMAHA中进行三维（3D）培养的IFPSC进行评估，以测定其增殖和分化为软骨细胞的能力。结果显示COL2和ACN的基因表达增加，COL1表达减少。DCP/AHAMA提供了一个模拟天然软骨生物力学特性的微环境，促进软骨生成分化，阻止肥大，并证明其适用于软骨组织工程以及临床生物医学应用的潜力。
