Gao Leyi, Pulido Jose S, Hatfield R Mark, Dundervill Robert F, McCannel Colin A, Shippy Scott A
Department of Chemistry, University of Illinois at Chicago, 845 West Taylor Street, M/C 111, Chicago, IL 60607, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Mar 1;847(2):300-4. doi: 10.1016/j.jchromb.2006.10.023. Epub 2006 Nov 13.
The determination of nitric oxide (NO) in human vitreous samples is complicated by the relatively short half-life of the analyte and the viscous, high salt and protein biological matrix. In this work, we developed a fast (<5min) and useful CE method to determine the stable metabolite, nitrate, from vitreous samples. This proposed method has been successfully applied to determine the nitrate levels from the vitreous humor of patients undergoing vitrectomy for a variety of conditions. A statistically significant increase (P=0.000001) of the mean level of nitrate was observed in vitreous humor of patients with proliferative diabetic retinopathy (41.17+/-4.09microM, n=27) versus controls (15.22+/-0.86microM, n=35). The elevated levels of nitrate in the vitreous of patients known to have diabetic retinopathy suggests that NO is involved with the pathology of this disease.
人玻璃体样本中一氧化氮（NO）的测定因分析物相对较短的半衰期以及粘性、高盐和蛋白质生物基质而变得复杂。在这项工作中，我们开发了一种快速（<5分钟）且实用的毛细管电泳（CE）方法来测定玻璃体样本中的稳定代谢物硝酸盐。该方法已成功应用于测定因各种病症接受玻璃体切除术患者玻璃体液中的硝酸盐水平。与对照组（15.22±0.86微摩尔，n = 35）相比，增殖性糖尿病视网膜病变患者（41.17±4.09微摩尔，n = 27）的玻璃体液中硝酸盐平均水平出现了统计学上的显著升高（P = 0.000001）。已知患有糖尿病视网膜病变患者玻璃体内硝酸盐水平升高表明NO参与了该疾病的病理过程。
