Lu Miao-Jen, Pulido Jose S, McCannel Colin A, Pulido Jose E, Hatfield R Mark, Dundervill Robert F, Shippy Scott A
Department of Chemistry, University of Illinois at Chicago, 845 W Taylor St., Chicago, IL 60607, USA.
Exp Diabetes Res. 2007;2007:39765. doi: 10.1155/2007/39765.
Elevated glutamate is implicated in the pathology of PDR. The ability to rapidly assess the glutamate and amino acid content of vitreous provides a more complete picture of the chemical changes occurring at the diabetic retina and may lead to a better understanding of the pathology of PDR. Vitreous humor was collected following vitrectomies of patients with PDR and control conditions of macular hole or epiretinal membrane. A capillary electrophoresis method was developed to quantify glutamate and arginine. The analysis is relatively fast (<6 minutes) and utilizes a poly(ethylene)oxide and sodium dodecylsulfate run buffer. Both amino acid levels show significant increases in PDR patients versus controls and are comparable to other reports. The levels of vitreal glutamate vary inversely with the degree of observed hemorrhage. The results demonstrate a rapid method for assessment of a number of amino acids to characterize the chemical changes at the diabetic retina to better understand tissue changes and potentially identify new treatments.
谷氨酸水平升高与增殖性糖尿病视网膜病变的病理过程有关。快速评估玻璃体液中谷氨酸和氨基酸含量的能力，可以更全面地了解糖尿病视网膜发生的化学变化，并可能有助于更好地理解增殖性糖尿病视网膜病变的病理。在对患有增殖性糖尿病视网膜病变的患者以及黄斑裂孔或视网膜前膜等对照情况进行玻璃体切割术后，收集了玻璃体液。开发了一种毛细管电泳方法来定量谷氨酸和精氨酸。该分析相对较快（<6分钟），并使用聚环氧乙烷和十二烷基硫酸钠运行缓冲液。与对照组相比，增殖性糖尿病视网膜病变患者的这两种氨基酸水平均显著升高，且与其他报告结果相当。玻璃体液中谷氨酸的水平与观察到的出血程度呈负相关。结果表明，有一种快速方法可用于评估多种氨基酸，以表征糖尿病视网膜的化学变化，从而更好地理解组织变化并可能确定新的治疗方法。
