Li Ning, Su Yu-Hong, Xi Huan-Jiu, Ren Fu, Zhu Bao-Qin, Wen You-Feng
College of Basic Science, Jinzhou Medical College, Liaoning Province, Jinzhou 121001, China.
Yi Chuan. 2006 Nov;28(11):1361-4. doi: 10.1360/yc-006-1361.
The polymorphism distributions of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818 and FGA) were investigated in a Lhasa Tibetan population by multiplex PCR amplification using five fluorochromes (6FAM, VIC, NED, PET, LIZ). Gene frequency, discrimination power (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (EPP) were calculated, and all loci were tested for Hardy-Weinberg equilibrium. Results indicate that the gene frequency of these 15 STR loci is in Hardy-Weinberg equilibrium. The DP is at 0.7515 - 0.9599, H is at 0.5576 - 0.8538, PIC is at 0.5455 - 0.8458, and EPP is at 0.37550.8520. Cumulative DP of the 15 STR is 0.99999999, and cumulative EPP is 0.999999997. Therefore, these 15 STR loci can be used as genetic markers of in Lhasa Tibetan populations in anthropological studies, linkage analysis of genetic diseases, individual identification and paternity testing in forensic medicine.
采用5种荧光染料（6FAM、VIC、NED、PET、LIZ）通过多重PCR扩增技术，对拉萨藏族人群中15个STR基因座（D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、VWA、TPOX、D18S51、D5S818和FGA）的多态性分布进行了研究。计算了基因频率、鉴别力（DP）、杂合度（H）、多态性信息含量（PIC）和排除父权概率（EPP），并对所有基因座进行了哈迪-温伯格平衡检验。结果表明，这15个STR基因座的基因频率处于哈迪-温伯格平衡状态。DP为0.7515 - 0.9599，H为0.5576 - 0.8538，PIC为0.5455 - 0.8458，EPP为0.3755 - 0.8520。这15个STR的累积DP为0.99999999，累积EPP为0.999999997。因此，这15个STR基因座可作为拉萨藏族人群人类学研究、遗传病连锁分析、法医学个体识别和亲子鉴定的遗传标记。
