Xu De-Hui, Huang Chen, Liu Li-Ying, Song Tu-Sheng
Department of Genetics and Molecular Biology, College of Medicine of JiaoTong University, Xi'an 710061, China.
Yi Chuan. 2006 Nov;28(11):1457-61. doi: 10.1360/yc-006-1457.
RNA interference (RNAi), a conserved evolutionary mechanism widely found in living nature, is considered for defending the alien gene and virus infection. RNAi can induce gene silencing by prohibiting the translation of the target mRNA, which is specially recognized by the corresponding siRNA, and finally degraded by the RISC. So RNAi can be applied as a new technology tool for the functional gene research and gene therapy. But the effects of random designed siRNAs on gene silencing differ greatly from each other. The design of the special, highly efficient siRNA comes to be a crucial problem. This issue gives an overview about the new progress of the siRNA design principle.
RNA干扰（RNAi）是一种在自然界广泛存在的保守进化机制，被认为可抵御外源基因和病毒感染。RNAi可通过抑制靶mRNA的翻译诱导基因沉默，靶mRNA由相应的小干扰RNA（siRNA）特异性识别，最终被RNA诱导沉默复合体（RISC）降解。因此，RNAi可作为一种新技术工具用于功能基因研究和基因治疗。但随机设计的siRNA对基因沉默的效果差异很大。设计特异、高效的siRNA成为一个关键问题。本文综述了siRNA设计原则的新进展。
