Lugowski C, Romanowska E
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland.
FEMS Microbiol Lett. 1991 Jan 15;61(2-3):315-8. doi: 10.1016/0378-1097(91)90572-r.
The antigenic reactivity of both native and chemically modified enterobacterial common antigen (ECA) with anti-ECA-tetanus toxoid (TT) conjugate serum was investigated. The results obtained suggest that reduction of the carboxyl group of the mannosaminuronic acid component of ECA diminishes, but does not destroy its antigenic reactivity. Each of the sugar components were found to contribute to its reactivity with anti-ECA-TT conjugate serum, indicating that the trisaccharide repeating unit represents the ECA epitope. A nonasaccharide (trimer of the ECA repeating unit) inhibited antibody binding better than the hexasaccharide dimer, a finding which suggests that oligosaccharide conformation also makes a contribution to its inhibitory activity.
研究了天然和化学修饰的肠杆菌共同抗原（ECA）与抗ECA - 破伤风类毒素（TT）偶联血清的抗原反应性。所得结果表明，ECA中甘露糖胺糖醛酸成分的羧基还原会降低但其抗原反应性并未破坏。发现每种糖成分都对其与抗ECA - TT偶联血清的反应性有贡献，这表明三糖重复单元代表了ECA表位。一种九糖（ECA重复单元的三聚体）比六糖二聚体能更好地抑制抗体结合，这一发现表明寡糖构象也对其抑制活性有贡献。
