Marusić-Galesić S, Pavelić K, Pokrić B
Ruder Bosković, Institute, Zagreb, Croatia, Yugoslavia.
Immunology. 1991 Apr;72(4):526-31.
Immune complexes are specifically bound to the Fc receptors on the surface of various types of cells capable of presenting antigens. It was therefore determined if human serum albumin (HSA), bound in an immune complex, is presented more efficiently to the HSA-specific T cells than HSA alone. Primed, polyclonal murine T cells were stimulated in vitro with either HSA alone or HSA bound at equivalence to syngeneic, polyclonal anti-HSA antibodies of IgG class. The stoichiometric composition of the insoluble immune complex was AgAb2.91. The present study shows that 10(2)-10(3)-fold lower doses of HSA are sufficient for the same T-cell response in vitro, if HSA is administered to the cultures in the form of the immune complex rather than in the soluble form. The enhancement of T-cell proliferation in the presence of immune complex was blocked with monoclonal antibody (mAb) against Fc receptor. Our results indicate that binding of antigen in the immune complex could play an important role in enhancing an antigen-specific cellular immune response.
免疫复合物会特异性地结合到能够呈递抗原的各类细胞表面的Fc受体上。因此，研究人员测定了免疫复合物中结合的人血清白蛋白（HSA）是否比单独的HSA更有效地呈递给HSA特异性T细胞。用单独的HSA或与同基因的IgG类多克隆抗HSA抗体等量结合的HSA在体外刺激已致敏的多克隆小鼠T细胞。不溶性免疫复合物的化学计量组成为AgAb2.91。本研究表明，如果以免疫复合物的形式而非可溶性形式将HSA加入培养物中，那么在体外只需10² - 10³倍低剂量的HSA就能引发相同的T细胞反应。针对Fc受体的单克隆抗体（mAb）可阻断免疫复合物存在时T细胞增殖的增强。我们的结果表明，免疫复合物中抗原的结合在增强抗原特异性细胞免疫反应中可能起重要作用。
