Murakami Y, Yasuda N, Saito Y, Nin H, Nakai S
Department of Otolaryngology, Kyoto Prefectural University of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1991 Feb;94(2):183-91. doi: 10.3950/jibiinkoka.94.183.
In order to obtain a more objective method to evaluate epithelial disorders and carcinomas of the hypopharynx, the expression pattern of cytokeratins (CKs) was investigated by ABC technique using several kinds of monoclonal antibodies that react monospecifically with each subclass of CKs. In normal epithelia, CK-19 was strongly positive in the basal layer but apparently reduced in suprabasal layers and completely negative in superficial layers, while CK-13 showed a striking contrast to CK-19, being expressed within the whole thickness of epithelia except only in the basal layer. These 2 subclasses were also observed in "abnormal" epithelia, and characteristic changes of their combination were demonstrated in proportion to the histological gradings. In invasive carcinomas, CK-19 was strongly positive in all carcinoma cells of poorly differentiated carcinomas. It was sporadically positive in moderately differentiated carcinomas, the more differentiated the more sporadic. It was completely negative in well differentiated carcinomas. CK-13, on the other hand, was negative in poorly differentiated carcinomas but positive in keratinized cells of moderately or well differentiated carcinomas. Strong expression of CK-1 was observed only in well keratinized cells of hyperkeratotic epithelia and well differentiated carcinomas. These characteristic findings are consistently observed in all samples and, then, may be useful in evaluating epithelial disorders and carcinomas of the hypopharynx, when used in conjunction with standard histological techniques. It seems most likely that these results play a part in investigating normal and abnormal processes of cell differentiation.
为了获得一种更客观的方法来评估下咽上皮紊乱和癌，利用几种与细胞角蛋白（CKs）各亚类发生单特异性反应的单克隆抗体，通过ABC技术研究了CKs的表达模式。在正常上皮中，CK-19在基底层呈强阳性，但在上基底层明显减弱，在表层完全阴性，而CK-13与CK-19形成鲜明对比，除基底层外，在上皮全层均有表达。在“异常”上皮中也观察到这两个亚类，并且它们组合的特征性变化与组织学分级成比例。在浸润性癌中，CK-19在低分化癌的所有癌细胞中呈强阳性。在中分化癌中呈散在阳性，分化程度越高越散在。在高分化癌中完全阴性。另一方面，CK-13在低分化癌中阴性，但在中分化或高分化癌的角化细胞中阳性。仅在角化过度上皮的高度角化细胞和高分化癌中观察到CK-1的强表达。在所有样本中均一致观察到这些特征性发现，因此，当与标准组织学技术结合使用时，可能有助于评估下咽上皮紊乱和癌。这些结果很可能在研究细胞分化的正常和异常过程中发挥作用。
