Saito Y
Department of Otolaryngology, Kyoto Prefectural University of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1994 Jul;97(7):1200-6. doi: 10.3950/jibiinkoka.97.1200.
The expression of cytokeratin (CK) subclasses was immunohistologically investigated in normal laryngeal epithelia by the ABC technique using monospecific monoclonal antibodies. There are two types of epithelium in the larynx; squamous epithelium of the glottis and ciliated epithelium mainly of the supraglottis. A difference in expression pattern was observed between these two epithelia only in 3 CKs, specifically CK-8, CK-13 and CK-19. In the glottis, CK-8 was negative in all layers, CK-13 was positive in the suprabasal and superficial layers, and CK-19 was strongly positive in the basal layer, but apparently reduced in suprabasal layers and completely negative in the superficial layers. In the supraglottis, on the contrary, CK-8 was positive except in the basal layer, CK-13 was negative in all layers, and CK-19 was positive in all layers. When ciliated epithelia were reduced to squamous metaplasia, the epithelial cells were morphologically similar to the squamous cells, and the CK expression also showed the same pattern. In proximity to this squamous metaplasia, however, there were lesions whose cell type morphologically still resembled that of the ciliated epithelium, but whose pattern of CK expression had already been reduced to that of the squamous cell.
采用抗细胞角蛋白（CK）亚类的单特异性单克隆抗体，运用ABC技术对正常喉上皮组织中的CK亚类表达进行免疫组织学研究。喉内存在两种上皮组织，即声门的鳞状上皮和主要位于声门上区的纤毛上皮。仅在3种细胞角蛋白（CK-8、CK-13和CK-19）中观察到这两种上皮组织的表达模式存在差异。在声门，CK-8在各层均为阴性，CK-13在基底上层和表层为阳性，CK-19在基底层呈强阳性，但在基底上层明显减少，在表层完全阴性。相反，在声门上区，CK-8除基底层外均为阳性，CK-13在各层均为阴性，CK-19在各层均为阳性。当纤毛上皮发生鳞状化生时，上皮细胞在形态上与鳞状细胞相似，CK表达也呈现相同模式。然而，在这种鳞状化生附近，存在一些病变，其细胞类型在形态上仍类似于纤毛上皮，但CK表达模式已降至鳞状细胞的表达模式。
