Murakami Y, Saito Y
Department of Otolaryngology, Kyoto Prefectural University of Medicine, Japan.
Ann Otol Rhinol Laryngol. 1990 Oct;99(10 Pt 1):782-8. doi: 10.1177/000348949009901005.
Expression of cytokeratins (CKs) was investigated immunohistochemically by use of monospecific monoclonal anti-CK antibodies in normal epithelia of pyriform sinus and epithelial lesions such as simple hyperplasia, different degrees of dysplasia, in situ carcinoma, and invasive carcinoma. In normal epithelium, strong expression of CK-19 was consistently observed only in the basal layer as basic CK, while expression of CK-13 showed a completely reverse pattern, being expressed only in suprabasal layers as stratification-related CK. Characteristic changes in expression pattern of these two CKs were observed in accordance with the degree of epithelial disorders and differentiation of carcinoma. Cytokeratin 1, as keratinization-associated CK, was observed only in keratinized cells of hyperplastic epithelia and well-differentiated carcinomas. These findings may be useful in evaluating epithelial disorders and classifying carcinomas more objectively, and may assist earlier detection of carcinoma when used with standard histologic techniques.
通过使用单特异性单克隆抗细胞角蛋白（CK）抗体，采用免疫组织化学方法研究了梨状窦正常上皮以及诸如单纯性增生、不同程度发育异常、原位癌和浸润癌等上皮病变中细胞角蛋白（CKs）的表达情况。在正常上皮中，仅在基底层作为基础细胞角蛋白持续观察到CK-19的强表达，而CK-13的表达模式则完全相反，仅在基底上层作为与分层相关的细胞角蛋白表达。根据上皮紊乱程度和癌的分化情况，观察到这两种细胞角蛋白表达模式的特征性变化。细胞角蛋白1作为与角化相关的细胞角蛋白，仅在增生上皮的角化细胞和高分化癌中观察到。这些发现可能有助于更客观地评估上皮紊乱和对癌进行分类，并且与标准组织学技术一起使用时可能有助于早期发现癌症。
