Remy Ingrid, Michnick Stephen W
Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, Québec, H3C 3J7, Canada.
Nat Methods. 2006 Dec;3(12):977-9. doi: 10.1038/nmeth979. Epub 2006 Nov 12.
Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels. We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGFbeta signaling.
蛋白质片段互补分析（PCA）为研究体内和体外蛋白质-蛋白质相互作用的动力学提供了一种通用策略。PCA的全部潜力需要在亚内源性蛋白质表达水平上完全可逆且灵敏的分析方法。我们描述了一种基于海肾荧光素酶的新分析方法，该方法满足这些标准，证明了化学可逆性以及胰岛素和转化生长因子β信号传导关键相互作用的诱导和抑制。
