基于高斯荧光素酶的哺乳动物细胞培养支原体检测试验

Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture.

作者信息

Degeling M Hannah, Bovenberg M Sarah S, Tannous Marie, Tannous Bakhos A

机构信息

Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.

出版信息

Methods Mol Biol. 2014;1098:47-55. doi: 10.1007/978-1-62703-718-1_4.

DOI:10.1007/978-1-62703-718-1_4

PMID:24166367

Abstract

Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter.

摘要

哺乳动物细胞培养中的支原体污染是一个常见问题，会对实验数据产生严重影响，然而许多实验室并未进行定期检测。在本章中，我们描述了一种基于高斯荧光素酶报告基因生物发光特性的简单且灵敏的支原体检测方法。