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[用于通过聚合酶链反应检测高致病性禽流感病毒H5N1毒株的“Ptakh-Grip PLR”诊断试剂盒的研发]

[Development of diagnostic kit "Ptakh-Grip PLR" for detection of highly pathogenic strain of avian influenza virus H5N1 by polymerase chain reaction].

作者信息

Bisiuk I Iu, Mel'nichuk S D, Oblap R V, Spiridonov V G, Martynenko D L, Mel'nichuk M D, Lisovenko V T

出版信息

Mikrobiol Z. 2006 Jul-Aug;68(4):70-8.

PMID:17100331
Abstract

A diagnostic kit for detection of avian influenza virus by real-time polymerase chain reaction was developed. This kit allows to identify the influenza viruses type A and highly pathogenic strain of avian influenza virus H5N1. The diagnostic kit is universal and adopted for ABI PRISM SDS (Applied Biosystems), RotorGene (Corbett Research) and iQCycler (BioRad) PCR machines.

摘要

开发了一种用于通过实时聚合酶链反应检测禽流感病毒的诊断试剂盒。该试剂盒能够鉴定甲型流感病毒和禽流感病毒H5N1的高致病性毒株。该诊断试剂盒具有通用性，适用于ABI PRISM SDS（应用生物系统公司）、RotorGene（科比特研究公司）和iQCycler（伯乐公司）的PCR仪。

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