Scavo L M, Serrano J, Roth J, de Pablo F
Section on Receptors and Hormone Action, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Biochem Biophys Res Commun. 1991 May 15;176(3):1393-401. doi: 10.1016/0006-291x(91)90441-9.
The early expression of insulin and insulin-like growth factor I (IGF-I) in the chicken embryo suggests that these peptides play an important role in early development. The receptors for insulin and IGF-I, however, had not been studied at the molecular level in this model. We report two chicken sequences that, by comparison with known tyrosine kinases, appear to correspond to the tyrosine kinase domain of the insulin receptor homologue (CTK-1) and the IGF-I receptor homologue (CTK-2). Using reverse-transcription of RNA, amplification with the polymerase chain reaction (RT-PCR), and gene-specific hybridization, we demonstrate that the two genes, CTK-1 and CTK-2, are expressed in embryos at least as early as the blastoderm (Day 0), during neurulation (Day 1), and in early (Days 2-3) and late (Day 9) organogenesis.
胰岛素和胰岛素样生长因子I（IGF-I）在鸡胚中的早期表达表明，这些肽在早期发育中发挥着重要作用。然而，在该模型中尚未在分子水平上研究胰岛素和IGF-I的受体。我们报告了两个鸡序列，通过与已知的酪氨酸激酶比较，它们似乎对应于胰岛素受体同源物（CTK-1）和IGF-I受体同源物（CTK-2）的酪氨酸激酶结构域。通过RNA的逆转录、聚合酶链反应（RT-PCR）扩增和基因特异性杂交，我们证明CTK-1和CTK-2这两个基因至少在胚盘期（第0天）、神经胚形成期（第1天）以及早期（第2 - 3天）和晚期（第9天）器官发生期的胚胎中表达。
