Seidler Marc, Salvenmoser Stefanie, Müller Frank-Michael C
Department of Paediatrics, Paediatric Pulmonology and Infectious Diseases, University of Heidelberg, Im Neuenheimer Feld 153, D-69120 Heidelberg, Germany.
Int J Antimicrob Agents. 2006 Dec;28(6):568-73. doi: 10.1016/j.ijantimicag.2006.07.024. Epub 2006 Nov 13.
Long-term inserted and surgically implanted catheters can be colonised by Candida spp. Candida biofilms in vitro are often resistant to antifungal agents. The aim of this study was to investigate the in vitro activity of micafungin (MFG) against six Candida spp. biofilms on polystyrene (PS) and central venous catheter (CVC) sections. Safranin staining and differential interference contrast microscopy were used to demonstrate biofilm production. MFG activity was determined by the reduction in metabolic activity (%RMA) by tetrazolium reduction assay on both substrates. In vitro, Candida albicans, Candida parapsilosis, Candida glabrata, Candida tropicalis, Candida dubliniensis and Candida kefyr produced mature biofilms on PS and CVC sections. MFG was active against C. kefyr (0.5 microg/mL) and C. glabrata (<0.5 microg/mL) on PS. However, MFG displayed resistance (>16 microg/mL) against C. albicans, C. dubliniensis,C. tropicalis and C. parapsilosis. On CVC disks, MFG was active against C. glabrata (1 microg/mL) as well as C. parapsilosis and C. albicans (<0.5 microg/mL). MFG was resistant (>16 microg/mL) against C. dubliniensis, C. tropicalis and C. kefyr. MFG was active in vitro against all six Candida spp. on both substrates. However, MFG could not reduce the metabolic activity completely even at the highest concentration.
长期置入和外科植入的导管可能会被念珠菌属定殖。念珠菌生物膜在体外通常对抗真菌剂耐药。本研究的目的是调查米卡芬净（MFG）对聚苯乙烯（PS）和中心静脉导管（CVC）切片上六种念珠菌属生物膜的体外活性。使用番红染色和微分干涉相差显微镜来证明生物膜的形成。通过在两种底物上进行四氮唑还原试验测定代谢活性降低率（%RMA）来确定MFG的活性。在体外，白色念珠菌、近平滑念珠菌、光滑念珠菌、热带念珠菌、都柏林念珠菌和克菲念珠菌在PS和CVC切片上形成了成熟的生物膜。MFG对PS上的克菲念珠菌（0.5μg/mL）和光滑念珠菌（<0.5μg/mL）有活性。然而，MFG对白色念珠菌、都柏林念珠菌、热带念珠菌和近平滑念珠菌表现出耐药性（>16μg/mL）。在CVC圆盘上，MFG对光滑念珠菌（1μg/mL）以及近平滑念珠菌和白色念珠菌（<0.5μg/mL）有活性。MFG对都柏林念珠菌、热带念珠菌和克菲念珠菌耐药（>16μg/mL）。MFG在体外对两种底物上的所有六种念珠菌属均有活性。然而，即使在最高浓度下，MFG也不能完全降低代谢活性。
