BACKGROUND: The incidence of invasive candidiasis is increasing worldwide. However, the epidemiology, antifungal susceptibility, and virulence of spp. in most hospitals remain unclear. This study aimed to evaluate invasive candidiasis in a tertiary care hospital in Nanchang City, China. METHODS: MALDI-TOF MS and 18S rDNA ITS sequencing were used to identify strains. Randomly amplified polymorphic DNA analysis was used for molecular typing; biofilm production, caseinase, and hemolysin activities were used to evaluate virulence. The Sensititre™ YeastOne YO10 panel was used to examine antifungal susceptibility. Mutations in and the hotspot regions of of drug-resistant strains were sequenced to evaluate the possible mechanisms of antifungal resistance. RESULTS: We obtained 110 strains, which included 40 (36.36%), 37 C. (33.64%), 21 C. (19.09%), 9 C. (8.18%), 2 C. (1.82%), and 1 C. (0.91%) isolates. At a limiting point of 0.80, . isolates could be grouped into five clusters, . and . isolates into seven clusters, and . isolates into only one cluster comprising six strains by RAPD typing. Antifungal susceptibility testing revealed that the isolates showed the greatest overall resistance against fluconazole (6.36%), followed by voriconazole (4.55%). All . and . isolates exhibited 100% susceptibility to echinocandins (i.e., anidulafungin, caspofungin, and micafungin), whereas one . strain was resistant to echinocandins. The most common amino acid substitutions noted in our study was 132aa (Y132H, Y132F) in the azole-resistant strains. No missense mutation was identified in the hotpot regions of . Comparison of the selected virulence factors detectable in a laboratory environment, such as biofilm, caseinase, and hemolysin production, revealed that most isolates were caseinase and hemolysin producers with a strong activity (Pz < 0.69). Furthermore, had greater total biofilm biomass (average Abs = 0.712) than (average Abs = 0.214, p < 0.01) or (average Abs = 0.450, < 0.05), although all strains were either low- or no-biofilm producers. The virulence level of the isolates from different specimen sources or clusters showed no obvious correlation. Interesting, 75% of the from cluster F demonstrated azole resistance, whereas two azole-resistant strains belonged to the cluster Y. CONCLUSION: This study provides vital information regarding the epidemiology, pathogenicity, and antifungal susceptibility of spp. in patients admitted to Nanchang City Hospital.