Hibi T, Aiso S, Ishikawa M, Watanabe M, Yoshida T, Kobayashi K, Asakura H, Tsuru S, Tsuchiya M
Clin Exp Immunol. 1983 Oct;54(1):163-8.
The fluorescence activated cell sorter (FACS) was used for detecting circulating antibodies to the surface antigens on isolated colon epithelial cells (anti-colon antibodies) by indirect immunofluorescence. Anti-colon antibodies were found in the serum of 30 of 41 (73%) patients with ulcerative colitis. This incidence is much higher than one established in earlier reports by application of indirect immunofluorescence to colon tissue using the fluorescence microscope. The results suggest that FACS analysis is very useful for detecting antibodies to colon specific antigen.
荧光激活细胞分选仪（FACS）用于通过间接免疫荧光检测分离的结肠上皮细胞表面抗原的循环抗体（抗结肠抗体）。在41例溃疡性结肠炎患者中，有30例（73%）的血清中发现了抗结肠抗体。这一发生率远高于早期报告中通过荧光显微镜对结肠组织应用间接免疫荧光所确定的发生率。结果表明，FACS分析对于检测结肠特异性抗原的抗体非常有用。
