聚乙二醇辅助的DNA在有机溶剂中的增溶作用，用于制备可被细胞质特异性降解的聚乙二醇/DNA纳米凝胶。

PEG-assisted DNA solubilization in organic solvents for preparing cytosol specifically degradable PEG/DNA nanogels.

作者信息

Mok Hyejung, Park Tae Gwan

机构信息

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea.

出版信息

Bioconjug Chem. 2006 Nov-Dec;17(6):1369-72. doi: 10.1021/bc060119i.

DOI:10.1021/bc060119i

PMID:17105212

Abstract

DNA was dissolved in selected organic solvents in the presence of poly(ethylene glycol) (PEG). Nanoscale PEG/DNA complex (approximately 100 nm) was produced in dimethylsulfoxide (DMSO) phase. Using a thiol-functionalized six-arm branched PEG for DNA solubilization, the PEG/DNA nanocomplex was cross-linked through the formation of disulfide linkages between the thiol groups, resulting in the production of stable PEG/DNA nanogels in aqueous solution. DNA release from the nanogels could be modulated by changing the concentration of an external reducing agent. The released plasmid DNA from the nanogels maintained intact structural integrity and exhibited appreciable gene transfection efficiency. The PEG/DNA nanogels could be potentially applied for gene therapy including DNA vaccination.

摘要

在聚乙二醇（PEG）存在的情况下，将DNA溶解于选定的有机溶剂中。在二甲基亚砜（DMSO）相中制备了纳米级PEG/DNA复合物（约100纳米）。使用硫醇功能化的六臂支化PEG使DNA增溶，PEG/DNA纳米复合物通过硫醇基团之间形成二硫键而交联，从而在水溶液中产生稳定的PEG/DNA纳米凝胶。纳米凝胶中DNA的释放可通过改变外部还原剂的浓度来调节。从纳米凝胶中释放的质粒DNA保持完整的结构完整性，并表现出可观的基因转染效率。PEG/DNA纳米凝胶可能潜在地应用于包括DNA疫苗接种在内的基因治疗。