Wilson B, Lea M A, Vidali G, Allfrey V G
Cancer Res. 1975 Nov;35(11 Pt 1):2954-8.
The contributions of nuclear populations to the total profile of nuclear proteins in a tissue were examined in normal rat liver and Morris hepatoma 7777. Comparison by sodium dodecyl sulfate polyacrylamide gel electrophoresis of phenol-soluble nuclear proteins from tumor and control liver revealed additional proteins of molecular weight 60,000, 100,00, and 135,000 and the loss of proteins of about 45,000 and 55,000 in the tumor. Subfractionation of liver nuclei on a 30 to 50% sucrose gradient yielded three nuclear classes with nearly identical complements of the phenol-soluble proteins. Similar fractionation performed on the hepatoma nuclei also produced three nuclear populations. In the hepatoma nuclei, several differences in the phenol-soluble proteins were found between the minor, slowly sedimenting nuclear fraction, and the two major fractions, while the two latter fractions were very similar in their protein composition. Histones derived from both tissues were also compared electrophoretically, indicating a decrease in the concentration of histone H1(0)in all nuclear classes derived from the tumor.
在正常大鼠肝脏和莫里斯肝癌7777中，研究了核群体对组织中核蛋白总谱的贡献。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳对肿瘤和对照肝脏中酚溶性核蛋白进行比较，发现肿瘤中有分子量为60,000、100,000和135,000的额外蛋白质，以及约45,000和55,000的蛋白质缺失。在30%至50%的蔗糖梯度上对肝细胞核进行亚分级分离，得到了三类核，其酚溶性蛋白质的组成几乎相同。对肝癌细胞核进行类似的分级分离也产生了三类核群体。在肝癌细胞核中，在较小的、沉降缓慢的核级分与两个主要级分之间发现了酚溶性蛋白质的几个差异，而后两个级分的蛋白质组成非常相似。还对来自两种组织的组蛋白进行了电泳比较，表明肿瘤来源的所有核类中组蛋白H1(0)的浓度降低。
