Kuz'mina S N, Bul'diaeva T V, Zbaraskiĭ I B
Biokhimiia. 1980 Aug;45(8):1417-24.
A comparative study of the nuclear matrix proteins of rat liver and Zajdela hepatoma cells was performed. The polyacrylamide SDS electrophoretic profile of the hepatoma nuclear matrix proteins differed from those of the liver by the presence of high molecular weight (over 135 KD) bands. Four nuclear matrix fractions were isolated by a subsequent treatment of the preparation with an aqueous solution of EDTA and 0,025 N sodium hydroxide. The bulk of the nuclear matrix proteins of both liver and hepatoma were alkali-soluble. The percentage of the alkali-insoluble residue and of the water-soluble fraction in the Zajdela hepatoma nuclear matrix was 3.5 and 1.7 times that of the liver, respectively. In the course of 60 min incubation of the liver mince or Zajdela hepatoma cells with 14C-Chlorella protein hydrolyzate in vitro the nuclear matrix proteins incorporated by 10-20% more label than did the total nuclear protein, the specific activity of the alkali-insoluble residue being twice higher that of the whole nuclear matrix protein. After 15 min of incubation the label was rather evenly spread along the gel, containing labelled protein bands separated according to their molecular weight. However, after 30 min and especially 60 min of incubation the label markedly prevailed in the high molecular weight proteins.
对大鼠肝脏和Zajdela肝癌细胞的核基质蛋白进行了一项比较研究。肝癌细胞核基质蛋白的聚丙烯酰胺SDS电泳图谱与肝脏的不同，在于存在高分子量（超过135KD）条带。通过用EDTA水溶液和0.025N氢氧化钠对制备物进行后续处理，分离出四个核基质组分。肝脏和肝癌的大部分核基质蛋白都是碱溶性的。Zajdela肝癌细胞核基质中碱不溶性残渣和水溶性组分的百分比分别是肝脏的3.5倍和1.7倍。在将肝脏切碎物或Zajdela肝癌细胞与14C-小球藻蛋白水解物在体外孵育60分钟的过程中，核基质蛋白比总核蛋白掺入的标记多10 - 20%，碱不溶性残渣的比活性是整个核基质蛋白的两倍。孵育15分钟后，标记相当均匀地分布在凝胶上，凝胶上含有根据分子量分离的标记蛋白条带。然而，孵育30分钟尤其是60分钟后，标记明显在高分子量蛋白中占优势。
