Ruoslahti E, Oh E, Jalanko H
Oncodev Biol Med. 1980 Aug;1(1):17-26.
Immunological tests and 2-dimensional gel electrophoresis revealed differences between the nuclear proteins from normal and malignant liver cells. Immunization of rabbits with a nuclear nonhistone protein fraction isolated from normal mouse liver resulted in antisera that in immunofluorescence gave nuclear staining in sections of normal liver, but did not stain nuclei of a transplantable mouse hepatoma. Antisera prepared against a nuclear protein fraction from the hepatoma allowed immunofluorescent staining of hepatoma nuclei but did not stain nuclei from normal liver. Nuclei in liver regeneration after injury caused by administration of carbon tetrachloride stained with antiserum against normal nuclei but not with antisera against hepatoma nuclei. Neither antiserum stained nuclei in fetal liver cells. The presence of differing sets of nuclear proteins in the normal and malignant liver cells was indicated by 2-dimensional gel electrophoresis. Approximately 300 polypeptides were resolved by this technique. Most of these were quantitatively different in the normal and hepatoma nonhistone protein preparations. Several qualitative differences also seemed to be present. Nonhistone proteins from regenerating liver shared some polypeptides with hepatoma that were absent in normal liver. Nuclear proteins characteristic of malignant tissues may provide clues toward the understanding of gene regulation in malignant cells, and some of them could become useful tumor markers.
免疫检测和二维凝胶电泳显示正常肝细胞与恶性肝细胞的核蛋白存在差异。用从正常小鼠肝脏分离的核非组蛋白蛋白组分免疫兔子，产生的抗血清在免疫荧光检测中可使正常肝脏切片的细胞核染色，但不能使可移植小鼠肝癌细胞核染色。用肝癌核蛋白组分制备的抗血清可使肝癌细胞核进行免疫荧光染色，但不能使正常肝脏细胞核染色。四氯化碳给药造成损伤后肝脏再生过程中的细胞核，能用抗正常细胞核的抗血清染色，而不能用抗肝癌细胞核的抗血清染色。两种抗血清均不能使胎儿肝细胞的细胞核染色。二维凝胶电泳表明正常肝细胞与恶性肝细胞中存在不同组别的核蛋白。用该技术可分辨出大约300种多肽。其中大多数在正常和肝癌非组蛋白蛋白制剂中的含量存在差异。似乎也存在一些定性差异。再生肝脏的非组蛋白蛋白与肝癌有一些正常肝脏中不存在的共同多肽。恶性组织特有的核蛋白可能为理解恶性细胞中的基因调控提供线索，其中一些可能成为有用的肿瘤标志物。
