安塔利亚真小鲤胃肠道内分泌细胞分布的免疫组织化学研究
Immunohistochemical study on distribution of endocrine cells in gastrointestinal tract of flower fish (Pseudophoxinus antalyae).
作者信息
Cinar Kenan, Senol Nurgül, Ozen M Rüştü
机构信息
Department of Biology, Süleyman Demirel University, Isparta 32260, Turkey.
出版信息
World J Gastroenterol. 2006 Nov 14;12(42):6874-8. doi: 10.3748/wjg.v12.i42.6874.
AIM
To detect distribution and relative frequency of endocrine cells in gastrointestinal tract of flower fish (Pseudophoxinus antalyae).
METHODS
The intestinal tract of flower fish was divided into four portions from proximal to distal; the enlarged area after oesophagus and anterior, middle and posterior intestine. Immunohistochemical method using the peroxidase anti-peroxidase complex was employed. All antisera between four portions of flower fish were compared using ANOVA.
RESULTS
Eleven types of gut endocrine cells were determined; they were immunoreactive for calcitonin gene related peptide, substance P, vasoactive intestinal peptide, bombesin, somatostatin-14, secretin, TrkA, TrkB, TrkC, neurotensin, neuropeptide Y, which were found in almost all portions of the gastrointestinal tract.
CONCLUSION
The regional distribution and relative frequency of immunoreactive cells in the flower fish, Pseudophoxinus antalyae, are essentially similar to those of other fish.
目的
检测安塔利亚真小鲤胃肠道内分泌细胞的分布及相对频率。
方法
将安塔利亚真小鲤的肠道从近端到远端分为四个部分，即食管后的扩大区域以及前肠、中肠和后肠。采用过氧化物酶抗过氧化物酶复合物免疫组织化学方法。使用方差分析比较安塔利亚真小鲤四个部分之间的所有抗血清。
结果
确定了11种肠道内分泌细胞；它们对降钙素基因相关肽、P物质、血管活性肠肽、蛙皮素、生长抑素-14、促胰液素、酪氨酸激酶A、酪氨酸激酶B、酪氨酸激酶C、神经降压素、神经肽Y呈免疫反应，几乎在胃肠道的所有部分都能发现。
结论
安塔利亚真小鲤免疫反应细胞的区域分布和相对频率与其他鱼类基本相似。
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