黄颡鱼消化道的免疫组织化学研究。
Immunohistochemical study of the digestive tract of Oligosarcus hepsetus.
机构信息
Departamento de Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23890-000, Brazil.
出版信息
World J Gastroenterol. 2013 Mar 28;19(12):1919-29. doi: 10.3748/wjg.v19.i12.1919.
Abstract
AIM
To describe the histology of the digestive tract and to investigate the occurrence of endocrine cells in Oligosarcus hepsetus (O. hepsetus).
METHODS
The digestive tract (DT) of O. hepsetus was divided into esophagus, two stomach regions (glandular and non-glandular) and two intestinal regions (anterior and posterior). These specimens were processed by routine histological techniques and stained with hematoxylin-eosin, Gomori's trichrome, periodic acid Schiff (PAS) and Alcian blue (AB). An immunohistochemical method using avidin-biotin-peroxidase was employed.
RESULTS
The esophagus is lined with a non-keratinized stratified squamous epithelium that is reactive to PAS and AB. The stomach has a mucosa lined with a simple columnar epithelium with mucus-secreting cells that are reactive only to PAS. The intestine has a simple columnar epithelium with a brush border and goblet cells that are reactive to PAS and AB. Somatostatin, serotonin and cholecystokinin immunoreactive cells were identified throughout the DT.
CONCLUSION
This study revealed adaptations for the species' diet and showed that the distribution and relative frequency of immunoreactive cells are similar to those of other fish.
摘要
目的
描述圆口铜鱼消化道的组织学结构，并研究其内分泌细胞的发生情况。
方法
将圆口铜鱼的消化道（DT）分为食管、两个胃区（腺胃和非腺胃）和两个肠区（前肠和后肠）。采用常规组织学技术对这些标本进行处理，并分别用苏木精-伊红、Gomori 三色、过碘酸雪夫（PAS）和阿利新蓝（AB）染色。采用亲和素-生物素-过氧化物酶免疫组织化学方法。
结果
食管被覆非角化复层扁平上皮，对 PAS 和 AB 呈反应性。胃的黏膜衬有单层柱状上皮，其中有分泌黏液的细胞，仅对 PAS 呈反应性。肠具有具有刷状缘的单层柱状上皮和杯状细胞，对 PAS 和 AB 均呈反应性。在 DT 中发现了生长抑素、血清素和胆囊收缩素免疫反应细胞。
结论
本研究揭示了该物种对其饮食的适应性，并表明免疫反应细胞的分布和相对频率与其他鱼类相似。
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