Dzagurov G K, Kazachkov Iu A, Kusov Iu Iu, Dzugkoeva F S
Lab Delo. 1990(12):77-8.
A modified technique of protein staining with silver nitrate was employed in electrophoretic analysis of hepatitis A virus structural proteins. The modifications of the original technique, i.e. thorough washing of the gel, increased formaldehyde concentration and a more intensive lighting of the gel, have elevated the method sensitivity 10-fold permitting the detection of up to 0.1 ng protein. The modification has allowed detection of VP1, VP2, and VP3 structural proteins with molecular masses 33, 29, and 27 kD, respectively, in virus preparations of low concentrations.
一种改良的硝酸银蛋白质染色技术被用于甲型肝炎病毒结构蛋白的电泳分析。对原始技术的改进，即对凝胶进行彻底洗涤、提高甲醛浓度以及对凝胶进行更强的光照，使该方法的灵敏度提高了10倍，能够检测低至0.1 ng的蛋白质。这种改进使得在低浓度病毒制剂中能够检测到分子量分别为33 kD、29 kD和27 kD的VP1、VP2和VP3结构蛋白。
