[甲型肝炎病毒结构蛋白的图谱分析]
[Mapping of the structural proteins of the hepatitis A virus].
作者信息
Kusov Iu Iu, Kazachkov Iu A, Dzagurov G K, Nastashenko T A, Balaian M S, Kozhich A T
出版信息
Vopr Virusol. 1990 Nov-Dec;35(6):468-71.
PMID:1964513
Abstract
The Western blot procedure with highly specific antipeptide antibody was applied to identify the electrophoretic mobility of hepatitis A virus capsid proteins. Polypeptides with molecular weights of 33, 29 and 27 kDa proved to be VP1, VP2, and VP3 proteins as they reacted with sera generated to VP1 recombinant protein and to synthetic oligopeptides 42-62 VP2 and 62-75 VP3, respectively.
摘要
采用具有高度特异性的抗肽抗体的蛋白质印迹法来鉴定甲型肝炎病毒衣壳蛋白的电泳迁移率。分子量为33、29和27 kDa的多肽被证明分别是VP1、VP2和VP3蛋白，因为它们分别与针对VP1重组蛋白以及合成寡肽42 - 62 VP2和62 - 75 VP3产生的血清发生反应。
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