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鸟类血细胞的改良染色技术。

Modified staining techniques for avian blood cells.

作者信息

Robertson G W, Maxwell M H

机构信息

Agricultural and Food Research Council Institute of Animal Physiology and Genetics Research, Roslin, Midlothian, Scotland.

出版信息

Br Poult Sci. 1990 Dec;31(4):881-6. doi: 10.1080/00071669008417319.

DOI:10.1080/00071669008417319
PMID:1710945
Abstract
  1. Two routine staining methods for domestic fowl blood cells have been modified with superior results. 2. Type of anticoagulant had a major effect on staining quality: EDTA gave excellent results whereas lithium heparin was unsatisfactory. 3. Unfixed blood smears were preferred to smears fixed in methanol before staining with May Grunwald and Giemsa's stain. Intact heterophil and basophil granules were clearly demonstrated. 4. Staining for 60 min in Natt and Herrick's haemocytometer diluent improved the differentiation between small lymphocytes and thrombocytes.
摘要
  1. 两种家鸡血细胞常规染色方法已被改良,效果更佳。2. 抗凝剂类型对染色质量有重大影响:乙二胺四乙酸(EDTA)效果极佳,而肝素锂则不尽人意。3. 在使用美-姬氏染色法染色前,未固定的血涂片比用甲醇固定的涂片更受青睐。完整的异嗜性粒细胞和嗜碱性粒细胞颗粒清晰可见。4. 在纳特和赫里克血细胞计数器稀释液中染色60分钟,改善了小淋巴细胞和血小板之间的鉴别。

相似文献

1
Modified staining techniques for avian blood cells.鸟类血细胞的改良染色技术。
Br Poult Sci. 1990 Dec;31(4):881-6. doi: 10.1080/00071669008417319.
2
Importance of optimal mixtures of EDTA anticoagulant:blood for the preparation of well-stained avian blood smears.乙二胺四乙酸(EDTA)抗凝剂与血液的最佳混合比例对于制备染色良好的禽类血涂片的重要性。
Br Poult Sci. 1993 Jul;34(3):615-7. doi: 10.1080/00071669308417617.
3
Effects of lithium heparin and tripotassium EDTA on hematologic values of Hermann's tortoises (Testudo hermanni).肝素锂和乙二胺四乙酸三钾对赫尔曼陆龟(Testudo hermanni)血液学指标的影响。
J Zoo Wildl Med. 1998 Mar;29(1):40-4.
4
Differentiation of avian thrombocytes from leukocytes by use of Giemsa's stain.利用吉姆萨染色法从白细胞中鉴别禽类血小板。
Poult Sci. 1967 Nov;46(6):1595-6. doi: 10.3382/ps.0461595.
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A new stain for identification of avian leukocytes.一种用于鉴定禽类白细胞的新型染色剂。
Biotech Histochem. 2002 Jul;77(4):201-6.
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Comparative analysis of different flow cytometry-based immunophenotypic methods for the analysis of CD59 and CD55 expression on major peripheral blood cell subsets.基于流式细胞术的不同免疫表型方法对主要外周血细胞亚群上CD59和CD55表达分析的比较研究
Cytometry. 2002 Jun 15;50(3):191-201. doi: 10.1002/cyto.10072.
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Morphologic, cytochemical staining, and ultrastructural characteristics of blood cells from eastern diamondback rattlesnakes (Crotalus adamanteus).东部菱斑响尾蛇(Crotalus adamanteus)血细胞的形态学、细胞化学染色及超微结构特征
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The development of eosinophils in the bone marrow of the fowl and the duck.家禽和鸭骨髓中嗜酸性粒细胞的发育。
J Anat. 1978 Feb;125(Pt 2):387-400.
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Topochemistry of blood cells of the Gallus domesticus (Aves, Galliforme).家鸡(鸟纲,鸡形目)血细胞的拓扑化学
Rev Bras Biol. 1991 Feb;51(1):211-4.
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[Microscopic diagnosis of Pneumocystis carinii. Comparison of various staining technics].卡氏肺孢子虫的显微镜诊断。各种染色技术的比较
G Batteriol Virol Immunol. 1985 Jul-Dec;78(7-12):178-84.

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