乙二胺四乙酸（EDTA）抗凝剂与血液的最佳混合比例对于制备染色良好的禽类血涂片的重要性。
Importance of optimal mixtures of EDTA anticoagulant:blood for the preparation of well-stained avian blood smears.
作者信息
Robertson G W, Maxwell M H
机构信息
AFRC Roslin Institute (Edinburgh), Roslin, Midlothian, Scotland.
出版信息
Br Poult Sci. 1993 Jul;34(3):615-7. doi: 10.1080/00071669308417617.
PMID:7689415
Abstract
- This paper describes and illustrates the dangers of sub-optimal mixtures of EDTA anticoagulant:blood during the preparation of avian blood smears. 2. Excessive concentrations of EDTA lead to variable staining and make cellular differentiation more difficult. 3. It is recommended, for the use of a 2.5 ml graded blood tube containing 3.75 mg EDTA, that no less than 1.0 ml of blood be mixed.
摘要
- 本文描述并说明了在制备禽类血涂片时，乙二胺四乙酸（EDTA）抗凝剂与血液的配比欠佳所带来的风险。2. EDTA浓度过高会导致染色不均，增加细胞分化的难度。3. 对于使用装有3.75毫克EDTA的2.5毫升刻度血样管，建议加入不少于1.0毫升血液进行混合。
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