Nasoff M S, Zebedee S L, Inchauspé G, Prince A M
Pharmacia Genetic Engineering, Inc., La Jolla, CA 92037.
Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5462-6. doi: 10.1073/pnas.88.12.5462.
We have isolated cDNA clones from the 5' end of the Hutchinson strain of hepatitis C virus. Sequences encoding various segments of the HCV structural region were fused to the gene for glutathione S-transferase and analyzed for the expression of hepatitis C virus-capsid fusion proteins. With a set of these fusion proteins, both human and chimpanzee immune responses to capsid were studied. An immunodominant epitope was located within the amino-terminal portion of capsid that is preferentially recognized by antibodies in both human and chimpanzee hepatitis C virus-positive sera. In addition, analyses of sequential serum samples taken from humans and chimpanzees with either chronic or apparently self-limited infections revealed that a strong anti-capsid response develops rapidly after onset of infection.
我们已从丙型肝炎病毒哈钦森株的5'端分离出cDNA克隆。将编码丙型肝炎病毒结构区各个片段的序列与谷胱甘肽S-转移酶基因融合，并分析丙型肝炎病毒衣壳融合蛋白的表达。利用一组这些融合蛋白，研究了人类和黑猩猩对衣壳的免疫反应。一个免疫显性表位位于衣壳的氨基末端部分，在人类和黑猩猩丙型肝炎病毒阳性血清中优先被抗体识别。此外，对患有慢性或明显自限性感染的人类和黑猩猩的连续血清样本分析显示，感染开始后很快就会产生强烈的抗衣壳反应。
