Johnson M A, Misra R M, Lardelli M, Messina M, Ephraums C, Reeves P R, Bolcevic Z, Noel J S, Hum C P, Van Mai H
Department of Microbiology, University of Sydney, N.S.W., Australia.
Gene. 1989 Dec 7;84(1):73-81. doi: 10.1016/0378-1119(89)90141-8.
Various regions of the gene encoding the major neutralization antigen, VP7, of human rotavirus have been expressed in Escherichia coli, as N-terminal fusions to beta-galactosidase under the control of the lac promoter. We have determined that the fusion products of two clones containing regions AB (aa 69-158) and ABC (aa 69-319) were antigenic, reacting with antibodies raised against whole virus. When guinea pigs were immunized with fusion protein purified by monoclonal antibody affinity columns, no neutralizing or virus-binding antibodies were detected, but antibodies binding to denatured VP7 were present.
