采用酶联免疫吸附测定（ELISA）技术测定近期酒精摄入量的标志物——葡萄糖醛酸化5-羟色醇（GTOL）。
Determination of glucuronidated 5-hydroxytryptophol (GTOL), a marker of recent alcohol intake, by ELISA technique.
作者信息
Dierkes Jutta, Wolfersdorf Manfred, Borucki Katrin, Weinmann Wolfgang, Wiesbeck Gerhard, Beck Olof, Borg Stefan, Wurst Friedrich M
机构信息
Institute of Clinical Chemistry, Otto von Guericke University, Magdeburg, Germany.
出版信息
Clin Biochem. 2007 Jan;40(1-2):128-31. doi: 10.1016/j.clinbiochem.2006.10.010. Epub 2006 Oct 20.
OBJECTIVES
To compare markers of alcohol consumption.
DESIGN AND METHODS
Measurement of urinary ethyl glucuronide, 5-hydroxytryptophol, 5-hydroxytryptophol glucuronide, 5-hydroxyindole acetic acid in 10 patients during alcohol withdrawal.
RESULTS
5-Hydroxytryptophol glucuronide was measured by ELISA with good analytical precision, its diagnostic specificity and sensitivity was better than that of 5-hydroxytryptophol and its correlation was closer to ethyl glucuronide than to 5-hydroxytryptophol.
CONCLUSION
Determination of 5-hydroxytryptophol glucuronide by ELISA offers promising results in detection of previous alcohol consumption.
目的
比较酒精摄入的标志物。
设计与方法
对10例酒精戒断患者的尿中乙基葡萄糖醛酸、5-羟色醇、5-羟色醇葡萄糖醛酸、5-羟吲哚乙酸进行测定。
结果
采用酶联免疫吸附测定法（ELISA）测定5-羟色醇葡萄糖醛酸，分析精密度良好，其诊断特异性和敏感性优于5-羟色醇，与乙基葡萄糖醛酸的相关性比与5-羟色醇的相关性更紧密。
结论
采用ELISA法测定5-羟色醇葡萄糖醛酸在检测既往酒精摄入方面有良好前景。
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