Giulietti Annapaula, van Etten Evelyne, Overbergh Lut, Stoffels Katinka, Bouillon Roger, Mathieu Chantal
Laboratory of Experimental Medicine and Endocrinology, Catholic University of Leuven, Herestraat 49, Leuven, Belgium.
Diabetes Res Clin Pract. 2007 Jul;77(1):47-57. doi: 10.1016/j.diabres.2006.10.007. Epub 2006 Nov 16.
The exact factors contributing to the pathogenesis of type 2 diabetes remain elusive. Lately, it was suggested that inflammation and activation of the innate immune system could be linked to type 2 diabetes pathogenesis and also to the development of common diabetic complications, mainly atherosclerosis. The aim of this study was to investigate the role of monocytes in this sub-clinical inflammatory state and test 1,25-dihydroxyvitamin D(3), the active form of Vitamin D, as an anti-inflammatory agent. For this purpose, monocytes from type 2 diabetic patients were compared to monocytes from healthy controls and type 1 diabetic patients. The expression profile of inflammatory markers in freshly isolated and immune-stimulated monocytes was measured by quantitative real-time RT-PCR. Type 2 diabetic patients showed significantly higher expression levels of TNF-alpha, IL-6, IL-1, IL-8, COX-2, ICAM-1 and B7-1 compared to controls and type 1 diabetic patients. 1,25-Dihydroxyvitamin D(3) was able to down-regulate the expression of TNF-alpha, IL-6, IL-1, and IL-8, confirming its immunomodulatory properties. From these data we concluded that monocytes from type 2 diabetic patients have a pro-inflammatory profile. In addition, 1,25-dihydroxyvitamin D(3) was able to modulate inflammation in these monocytes.
导致2型糖尿病发病的确切因素仍不清楚。最近，有人提出炎症和先天性免疫系统的激活可能与2型糖尿病的发病机制有关，也与常见糖尿病并发症（主要是动脉粥样硬化）的发展有关。本研究的目的是调查单核细胞在这种亚临床炎症状态中的作用，并测试维生素D的活性形式1,25-二羟基维生素D(3)作为一种抗炎剂的效果。为此，将2型糖尿病患者的单核细胞与健康对照者和1型糖尿病患者的单核细胞进行比较。通过定量实时RT-PCR测量新鲜分离的和免疫刺激的单核细胞中炎症标志物的表达谱。与对照组和1型糖尿病患者相比，2型糖尿病患者的TNF-α、IL-6、IL-1、IL-8、COX-2、ICAM-1和B7-1表达水平显著更高。1,25-二羟基维生素D(3)能够下调TNF-α、IL-6、IL-1和IL-8的表达，证实了其免疫调节特性。从这些数据中我们得出结论，2型糖尿病患者的单核细胞具有促炎特征。此外，1,25-二羟基维生素D(3)能够调节这些单核细胞中的炎症。
