Arroyo Luis G, Staempfli Henry, Weese J Scott
Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ont., Canada N1G 2W1.
Vet Microbiol. 2007 Feb 25;120(1-2):179-83. doi: 10.1016/j.vetmic.2006.10.013. Epub 2006 Oct 20.
Clostridium difficile is an important cause of diarrhea in horses, causing sporadic and epidemic disease of varying severity. This study evaluated the molecular characteristics of 48 C. difficile isolates recovered from diarrheic horses admitted to a veterinary hospital by using PCR-ribotyping and toxin gene profile. Additionally, feces were tested for the presence of C. difficile toxin A/B via enzyme immunosorbant assay (EIA) in 38 horses. The toxin genes tcdA, tcdB and cdtB were present in 27 (56.25%), 35 (72.91%) and 2 (4.1%) strains, respectively. Eight isolates (16.6%) were A(-)B(+) variants. Thirteen of forty-eight isolates (27.0%) did not posses any toxin genes (A(-)B(-)CDT(-)). A positive EIA result was reported in 17 (44%) of the cases. There was no association between the presence of different ribotypes or strains and toxin gene(s) profiles and the clinical outcome.
艰难梭菌是马匹腹泻的重要病因，可引发不同严重程度的散发性和流行性疾病。本研究通过PCR核糖体分型和毒素基因谱分析，评估了从一家兽医医院收治的腹泻马匹中分离出的48株艰难梭菌的分子特征。此外，还对38匹马的粪便进行了酶免疫吸附测定（EIA），以检测艰难梭菌毒素A/B的存在。毒素基因tcdA、tcdB和cdtB分别存在于27株（56.25%）、35株（72.91%）和2株（4.1%）菌株中。8株分离株（16.6%）为A（-）B（+）变体。48株分离株中有13株（27.0%）不携带任何毒素基因（A（-）B（-）CDT（-））。17例（44%）病例的EIA结果呈阳性。不同核糖体分型或菌株的存在与毒素基因谱和临床结果之间无关联。
