Nagy Alexander, Machova Jirina, Hornickova Jitka, Tomci Miroslav, Nagl Ivan, Horyna Bedrich, Holko Ivan
State Veterinary Institute Prague, National Reference Laboratory for Avian Influenza, Sidlistni 136/24, 165 03 Prague 6, Czech Republic.
Vet Microbiol. 2007 Feb 25;120(1-2):9-16. doi: 10.1016/j.vetmic.2006.10.004. Epub 2006 Oct 12.
In order to determine the actual prevalence of avian influenza viruses (AIV) in wild birds in the Czech Republic extensive surveillance was carried out between January and April 2006. A total of 2101 samples representing 61 bird species were examined for the presence of influenza A by using PCR, sequencing and cultivation on chicken embryos. AIV subtype H5N1 was detected in 12 Mute swans (Cygnus olor). The viruses were determined as HPAI (highly pathogenic avian influenza) and the hemagglutinin sequence was closely similar to A/mallard/Italy/835/06 and A/turkey/Turkey/1194/05. Following the first H5N1 case, about 300 wild birds representing 33 species were collected from the outbreak region and tested for the presence of AIV without any positive result. This is the first report of highly pathogenic avian influenza subtype H5N1 in the Czech Republic. The potential role of swan as an effective vector of avian influenza virus is also discussed.
为确定捷克共和国野生鸟类中禽流感病毒（AIV）的实际流行情况，于2006年1月至4月期间开展了广泛监测。通过PCR、测序以及在鸡胚上培养，对代表61种鸟类的总共2101份样本进行了甲型流感检测。在12只疣鼻天鹅（Cygnus olor）中检测到了AIV H5N1亚型。这些病毒被确定为高致病性禽流感（HPAI），其血凝素序列与A/野鸭/意大利/835/06和A/火鸡/土耳其/1194/05密切相似。在首例H5N1病例出现后，从疫情爆发地区收集了代表33种鸟类的约300只野生鸟类，并检测是否存在AIV，但未得到任何阳性结果。这是捷克共和国高致病性禽流感H5N1亚型的首次报告。还讨论了天鹅作为禽流感病毒有效传播媒介的潜在作用。
