Kuraparthy Vasu, Sood Shilpa, Dhaliwal H S, Chhuneja Parveen, Gill Bikram S
Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502, USA.
Theor Appl Genet. 2007 Jan;114(2):285-94. doi: 10.1007/s00122-006-0431-y. Epub 2006 Nov 8.
Tillering is one of the most important agronomic traits in cereal crops because tiller number per plant determines the number of spikes or panicles per plant, a key component of grain yield and/or biomass. In order to characterize the underlying genetic variation for tillering, we have isolated mutants that are compromised in tillering ability using ethyl methanesulphonate (EMS)-based mutagenesis in diploid wheat (Triticum monococcum subsp. monococcum). The tillering mutant, tiller inhibition (tin3) produces only one main culm compared to the wild type with many tillers. The monoculm phenotype of tin3 is due to a single recessive mutation. Genetic and molecular mapping in an F(2) population of diploid wheat located the tin3 gene on the long arm of chromosome 3A(m). One codominant RFLP marker Xpsr1205 cosegregated with tin3 in the F(2) population. Physical mapping of PSR1205 in a set of Chinese Spring deletion lines of group-3 chromosomes placed the tin3 gene in the distal 10% of the long arm of chromosome 3A, which is a recombination-rich region in wheat. The implications of the mapping of tin3 on chromosome arm 3A(m)L are discussed with respect to putative orthologs of tin3 in the 3L colinear regions across various cereal genomes and other tillering traits in grasses.
分蘖是谷类作物最重要的农艺性状之一，因为单株分蘖数决定了单株穗数，而穗数是谷物产量和/或生物量的关键组成部分。为了表征分蘖潜在的遗传变异，我们利用基于甲磺酸乙酯（EMS）诱变的方法，在二倍体小麦（一粒小麦亚种一粒小麦）中分离出了分蘖能力受损的突变体。与具有多个分蘖的野生型相比，分蘖突变体“分蘖抑制”（tin3）只产生一个主茎。tin3的单茎表型是由一个隐性突变导致的。在二倍体小麦的F2群体中进行遗传和分子定位，将tin3基因定位在3A(m)染色体的长臂上。共显性RFLP标记Xpsr1205在F2群体中与tin3共分离。在一组中国春3组染色体缺失系中对PSR1205进行物理定位，将tin3基因定位在3A染色体长臂的远端10%区域，该区域是小麦中重组丰富的区域。我们讨论了tin3在3A(m)L染色体臂上的定位对于各种谷类基因组中3L共线区域内tin3的假定直系同源基因以及禾本科其他分蘖性状的意义。
