Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong, College of Agriculture, Ludong University, Yantai, 264025, People's Republic of China.
Crop Research Institute, Shandong Academy of Agricultural Science, Jinan, 250100, People's Republic of China.
Theor Appl Genet. 2024 Mar 21;137(4):87. doi: 10.1007/s00122-024-04589-x.
A total of 38 putative additive QTLs and 55 pairwise putative epistatic QTLs for tiller-related traits were reported, and the candidate genes underlying qMtn-KJ-5D, a novel major and stable QTL for maximum tiller number, were characterized. Tiller-related traits play an important role in determining the yield potential of wheat. Therefore, it is important to elucidate the genetic basis for tiller number when attempting to use genetic improvement as a tool for enhancing wheat yields. In this study, a quantitative trait locus (QTL) analysis of three tiller-related traits was performed on the recombinant inbred lines (RILs) of a mapping population, referred to as KJ-RILs, that was derived from a cross between the Kenong 9204 (KN9204) and Jing 411 (J411) lines. A total of 38 putative additive QTLs and 55 pairwise putative epistatic QTLs for spike number per plant (SNPP), maximum tiller number (MTN), and ear-bearing tiller rate (EBTR) were detected in eight different environments. Among these QTLs with additive effects, three major and stable QTLs were first documented herein. Almost all but two pairwise epistatic QTLs showed minor interaction effects accounting for no more than 3.0% of the phenotypic variance. The genetic effects of two colocated major and stable QTLs, i.e., qSnpp-KJ-5D.1 and qMtn-KJ-5D, for yield-related traits were characterized. The breeding selection effect of the beneficial allele for the two QTLs was characterized, and its genetic effects on yield-related traits were evaluated. The candidate genes underlying qMtn-KJ-5D were predicted based on multi-omics data, and TraesKN5D01HG00080 was identified as a likely candidate gene. Overall, our results will help elucidate the genetic architecture of tiller-related traits and can be used to develop novel wheat varieties with high yields.
共报道了 38 个与分蘖相关性状的假定加性 QTL 和 55 个两两假定上位性 QTL，并且对最大分蘖数的新主效和稳定 QTL qMtn-KJ-5D 的候选基因进行了鉴定。分蘖相关性状在决定小麦产量潜力方面起着重要作用。因此，在试图利用遗传改良作为提高小麦产量的工具时，阐明分蘖数的遗传基础非常重要。在这项研究中，对来自 Kenong 9204（KN9204）和 Jing 411（J411）杂交的重组自交系（RILs）群体 KJ-RILs 进行了三个分蘖相关性状的数量性状位点（QTL）分析。在八个不同环境中检测到了 38 个假定加性 QTL 和 55 个两两假定上位性 QTL 对单株穗数（SNPP）、最大分蘖数（MTN）和穗生分蘖率（EBTR）的影响。在具有加性效应的这些 QTL 中，首次记录了三个主效和稳定的 QTL。除了两个外，几乎所有的上位性 QTL 都表现出较小的互作效应，占表型方差的比例不超过 3.0%。对与产量相关性状的两个共定位主效和稳定 QTL qSnpp-KJ-5D.1 和 qMtn-KJ-5D 的遗传效应进行了分析。对两个 QTL 有益等位基因的育种选择效果进行了分析，并对其对产量相关性状的遗传效应进行了评价。基于多组学数据预测了 qMtn-KJ-5D 的候选基因，并鉴定出 TraesKN5D01HG00080 可能是候选基因。总的来说，我们的研究结果将有助于阐明分蘖相关性状的遗传结构，并可用于开发具有高产的新型小麦品种。
