Hantash Basil M, Bedi Vikramaditya P, Chan Kin Foong, Zachary Christopher B
Stanford University School of Medicine, Stanford, California 94305, USA.
Lasers Surg Med. 2007 Feb;39(2):87-95. doi: 10.1002/lsm.20405.
We introduce a novel CO(2) laser device that utilizes ablative fractional resurfacing for deep dermal tissue removal and characterize the resultant thermal effects in skin.
STUDY DESIGN/MATERIALS AND METHODS: A prototype 30 W, 10.6 microm CO(2) laser was focused to a 1/e(2) spot size of 120 microm and pulse duration up to 0.7 milliseconds to achieve a microarray pattern in ex vivo human skin. Lesion depth and width were assessed histologically using either hematoxylin & eosin (H&E) or lactate dehydrogenase (LDH) stain. Pulse energies were varied to determine their effect on lesion dimensions.
Microarrays of ablative and thermal injury were created in fresh ex vivo human skin irradiated with the prototype CO(2) laser device. Zones of tissue ablation were surrounded by areas of tissue coagulation spanning the epidermis and part of the dermis. A thin condensed lining on the interior wall of the lesion cavity was observed consistent with eschar formation. At 23.3 mJ, the lesion width was approximately 350 microm and depth 1 mm. In this configuration, the cavities were spaced approximately 500 microm apart and interlesional epidermis and dermis demonstrated viable tissue by LDH staining.
A novel prototype ablative CO(2) laser device operating in a fractional mode was developed and its resultant thermal effects in human abdominal tissue were characterized. We discovered that controlled microarray patterns could be deposited in skin with variable depths of dermal tissue ablation depending on the treatment pulse energy. This is the first report to characterize the successful use of ablative fractional resurfacing as a potential approach to dermatological treatment.
我们介绍一种新型二氧化碳激光设备，该设备利用剥脱性点阵激光技术去除深层真皮组织，并对皮肤中产生的热效应进行表征。
研究设计/材料与方法：一台30瓦、波长10.6微米的二氧化碳激光原型机聚焦至1/e²光斑尺寸为120微米，脉冲持续时间最长达0.7毫秒，以在离体人皮肤上形成微阵列模式。使用苏木精和伊红（H&E）染色或乳酸脱氢酶（LDH）染色，通过组织学方法评估损伤深度和宽度。改变脉冲能量以确定其对损伤尺寸的影响。
在用该二氧化碳激光原型机照射的新鲜离体人皮肤上形成了剥脱性和热损伤微阵列。组织消融区域被跨越表皮和部分真皮的组织凝固区域包围。在损伤腔内壁观察到一层薄的致密衬里，与焦痂形成一致。在23.3毫焦时，损伤宽度约为350微米，深度为1毫米。在这种配置下，腔隙间距约为500微米，损伤间的表皮和真皮经LDH染色显示为存活组织。
开发了一种以点阵模式运行的新型剥脱性二氧化碳激光原型机，并对其在人腹部组织中产生的热效应进行了表征。我们发现，根据治疗脉冲能量，可以在皮肤中形成可控的微阵列模式，并产生不同深度的真皮组织消融。这是第一篇描述成功使用剥脱性点阵激光技术作为一种潜在皮肤病治疗方法的报告。
