Carinci Francesco, Palmieri Annalisa, Perrotti Vittoria, Piattelli Adriano, Cenzi Roberto, Brunell Giorgio, Martinelli Marcella, Arlotti Marzia, Pezzetti Furio
Maxillofacial Surgery, University of Ferrara, Ferrara, Italy.
J Craniofac Surg. 2006 Nov;17(6):1243-50. doi: 10.1097/01.scs.0000248644.02718.28.
Porous polyethylene (PP or Medpor) is an alloplastic material used worldwide for craniofacial reconstruction. Although several clinical studies are available, there is a lack as regard the genetic effects. Because PP is always fixed on bone and the mechanism by which PP acts on osteoblasts is unknown, we therefore attempted to address this question by using microarray techniques to identify genes that are differently regulated in osteoblasts exposed to PP. By using DNA microarrays containing 19,200 genes, we identified in osteoblast-like cell lines (i.e. MG-63) cultured on PP several genes where expression was differentially regulated. The differentially expressed genes cover a broad range of functional activities: 1) signal transduction, 2) transcription, 3) translation, 4) cell cycle regulation, 5) vesicular transport, and 6) production of cytoskeletal elements, cell-adhesion molecules and extracellular matrix components. The data reported are, to our knowledge, the first genetic portrait of osteoblast-like cells cultured on PP. They are relevant to better understanding of the molecular mechanism of bone-PP interaction and as a model for comparing other materials used for bone reconstruction.
多孔聚乙烯（PP或Medpor）是一种在全球范围内用于颅面重建的异质材料。尽管有多项临床研究，但在遗传效应方面仍存在不足。由于PP总是固定在骨上，且PP作用于成骨细胞的机制尚不清楚，因此我们试图通过使用微阵列技术来鉴定在暴露于PP的成骨细胞中差异调节的基因，以解决这个问题。通过使用包含19200个基因的DNA微阵列，我们在PP上培养的成骨样细胞系（即MG-63）中鉴定出了几个表达差异调节的基因。差异表达的基因涵盖了广泛的功能活动：1）信号转导，2）转录，3）翻译，4）细胞周期调节，5）囊泡运输，以及6）细胞骨架元件、细胞粘附分子和细胞外基质成分的产生。据我们所知，所报道的数据是在PP上培养的成骨样细胞的首张遗传图谱。它们有助于更好地理解骨与PP相互作用的分子机制，并作为比较其他用于骨重建材料的模型。
