Nakano H, Sakaguchi G
Department of Food Microbiology and Hygiene, Hiroshima University, Japan.
FEMS Microbiol Lett. 1991 Apr 15;63(2-3):171-7. doi: 10.1016/0378-1097(91)90081-k.
Many spores (1-60/g) of Clostridium botulinum type F were detected in different containers of honey products of the same brand. Microbiological and physicochemical properties of the contaminated honey were compared with those of the negative one. No difference in pH, hydroxymethyl furfural contents or diastase activity was found between them. The total counts of anaerobes other than C. botulinum and of yeast were also similar, whereas the aerobe counts, which were proportionally related with the C. botulinum counts, were higher in the positive honey than in the negative one. Motile colony-forming Bacillus alvei was predominant among the aerobes. B. alvei stimulated the toxin production by C. botulinum type F in culture medium incubated under aerobic conditions. The high count of C. botulinum in the honey might have been due to the possible stimulation of growth by B. alvei or some other microorganisms at some stage of honey ripening.
在同一品牌的不同蜂蜜产品容器中检测到许多（1-60个/克）F型肉毒梭菌孢子。将受污染蜂蜜的微生物学和物理化学性质与未受污染的蜂蜜进行比较。两者之间在pH值、羟甲基糠醛含量或淀粉酶活性方面未发现差异。除肉毒梭菌外的厌氧菌总数和酵母总数也相似，而与肉毒梭菌数量成比例相关的需氧菌数量，阳性蜂蜜中的比阴性蜂蜜中的更高。运动性产菌落的蜂房芽孢杆菌在需氧菌中占主导地位。在有氧条件下培养的培养基中，蜂房芽孢杆菌刺激F型肉毒梭菌产生毒素。蜂蜜中肉毒梭菌数量高可能是由于在蜂蜜成熟的某个阶段，蜂房芽孢杆菌或其他一些微生物可能刺激了其生长。
