Department of Veterinary Sanitation, Faculty of Veterinary Medicine and Technology of Animal Husbandry, S. Seifullin Kazakh Agro Technical University, Zhenis Avenue 62, 010011 Astana, Republic of Kazakhstan.
Departament of Food Hygiene and Public Health Protection, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW (WULS-SGGW), ul.Nowoursynowska 159, 02-776 Warsaw, Poland.
Toxins (Basel). 2019 Aug 13;11(8):472. doi: 10.3390/toxins11080472.
The aim of this study was to assess occurrence of and in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of and . Among 197 samples, was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the and genes. strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to , which were and strains. prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and prevalence in Kazakh honey.
本研究旨在评估哈萨克斯坦蜂蜜样本中 和 的发生情况。分析使用了一组用于鉴定厌氧菌和检测 和 毒素基因的 PCR 方法。在 197 个样本中，仅在一个样本（0.5%）中发现了 。分离出的该病原体菌株显示存在 和 基因。从 18 个（9%）样本中分离出 菌株，多重 PCR（多重 PCR）分析将它们全部归类为具有产生α毒素能力的毒素型 A。16S rDNA 基因序列分析显示，在另外 4 个与 相关的其他厌氧菌样本中存在 ，分别为 和 菌株。与其他地区相比，哈萨克斯坦蜂蜜样本中的 流行率似乎较低。在东哈萨克斯坦省发现了最高的 sp. 流行率。我们的研究是首次对哈萨克蜂蜜中产 BoNT 的梭菌和 的流行率进行调查。
